SGD Paper 
Cogoni C, et al. (1995) Saccharomyces cerevisiae has a single glutamate synthase gene coding for a plant-like high-molecular-weight polypeptide. J Bacteriol 177(3):792-8
Abstract: Purification of the glutamate synthase (GOGAT) enzyme from Saccharomyces cerevisiae showed that it is an oligomeric enzyme composed of three identical 199-kDa subunits. The GOGAT structural gene was isolated by screening a yeast genomic library with a yeast PCR probe. This probe was obtained by amplification with degenerate oligonucleotides designed from conserved regions of known GOGAT genes. The derived amino-terminal sequence of the GOGAT gene was confirmed by direct amino-terminal sequence analysis of the purified protein of 199 kDa. Northern (RNA) analysis allowed the identification of an mRNA of about 7 or 8 kb. An internal fragment of the GOGAT gene was used to obtain null GOGAT mutants completely devoid of GOGAT activity. The results show that S. cerevisiae has a single NADH-GOGAT enzyme, consisting of three 199-kDa monomers, that differs from the one found in prokaryotic microorganisms but is similar to those found in other eukaryotic organisms such as alfalfa.
| Status: Published | Type: Journal Article | PubMed ID: 7836314 |
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| Topics | Genes linked to topics |
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| GLT1 | |
| DNA/RNA Sequence Features |  |
| Function/Process | |
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| Non-Fungal Related Genes/Proteins | |
| Primary Literature | |
| Protein Physical Properties | |
| RNA Levels and Processing | |
| Techniques and Reagents | |
