Rush K, et al. (1986) Null and electrophoretic mobility mutants in the structural gene for L-lactate dehydrogenase of Saccharomyces cerevisiae. FEBS Lett 198(1):89-91
Abstract: A mutant lacking L-lactate dehydrogenase (EC 18.104.22.168) of Saccharomyces cerevisiae was isolated by its inability to grow on minimal medium with L-lactate as a carbon source. A simple activity gel assay for visualization of this enzyme and the two D-lactate dehydrogenases in this organism (EC 22.214.171.124 and 126.96.36.199) was developed. This enabled us to screen spontaneous and ethylmethanesulfonate-induced back mutants for electrophoretic mobility. Two mutants with a mobility faster than that of the wild type were isolated, and proved to be allelic to the L-lactate dehydrogenase negative mutant.
|Status: Published||Type: Journal Article||PubMed ID: 3514273|
Topics addressed in this paper
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