Boeke JD, et al. (1987) 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol 154():164-75
Abstract: 5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.
|Status: Published||Type: Journal Article||PubMed ID: 3323810|
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