SGD Paper 
Chatton B, et al. (1987) Cloning and characterization of the yeast methionyl-tRNA synthetase mutation mes1. J Biol Chem 262(31):15094-7
Abstract: The chromosomal mes 1 mutation appears to elevate the Km of methionine for yeast methionyl-tRNA synthetase. The mutation was cloned on a multicopy plasmid by gap repair of a plasmid bearing the wild type MES1 gene for a fragment corresponding to the mes 1 mutation. DNA sequencing established that the mutation consists of a single conversion of guanine into adenine which results in the replacement of a glycine by an aspartic acid at position 502. This causes the enzyme to be labile and inactive in vitro and to show a requirement for high concentrations of methionine in vivo. The mutation is in the COOH-terminal domain of the mononucleotide binding fold of the yeast enzyme and suggests participation of this region in the binding of the amino acid residue.
| Status: Published | Type: Journal Article | PubMed ID: 3312199 |
Topics addressed in this paper
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| Topics | Genes linked to topics |
|---|---|
| MES1 | |
| DNA/RNA Sequence Features |  |
| Function/Process | |
| Mutants/Phenotypes | |
| Primary Literature | |
| Protein Sequence Features | |
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