De Graeve S, et al. (2013) Mammalian ribosomal and chaperone protein RPS3A counteracts a-synuclein aggregation and toxicity in a yeast model system. Biochem J 455(3):295-306
Abstract: Accumulation of aggregated forms of aSyn (a-synuclein) into Lewy bodies is a known hallmark associated with neuronal cell death in Parkinson's disease. When expressed in the yeast Saccharomyces cerevisiae, aSyn interacts with the plasma membrane, forms inclusions and causes a concentration-dependent growth defect. We have used a yeast mutant, cog6?, which is particularly sensitive to moderate aSyn expression, for screening a mouse brain-specific cDNA library in order to identify mammalian proteins that counteract aSyn toxicity. The mouse ribosomal and chaperone protein RPS3A was identified as a suppressor of aSyn [WT (wild-type) and A53T] toxicity in yeast. We demonstrated that the 50 N-terminal amino acids are essential for this function. The yeast homologues of RPS3A were not effective in suppressing the aSyn-induced growth defect, illustrating the potential of our screening system to identify modifiers that would be missed using yeast gene overexpression as the first screening step. Co-expression of mouse RPS3A delayed the formation of aSyn-GFP inclusions in the yeast cells. The results of the present study suggest that the recently identified extraribosomal chaperonin function of RPS3A also acts on the neurodegeneration-related protein aSyn and reveal a new avenue for identifying promising candidate mammalian proteins involved in aSyn functioning.
|Status: Published||Type: Journal Article||PubMed ID: 23924367|
Topics addressed in this paper
Number of different genes curated to this paper: 7
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