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Sikorski TW, et al.  (2012) Proteomic analysis demonstrates activator- and chromatin-specific recruitment to promoters. J Biol Chem 287(42):35397-408

Abstract: In-depth characterization of RNA polymerase II preinitiation complexes remains an important and challenging goal. We used quantitative mass spectrometry to explore context-dependent Saccharomyces cerevisiae preinitiation complex formation at the HIS4 promoter reconstituted on naked and chromatinized DNA templates. The transcription activators Gal4-VP16 and Gal4-Gcn4 recruited a limited set of chromatin-related coactivator complexes, namely the chromatin remodeler Swi/Snf and histone acetyltransferases SAGA and NuA4, suggesting that transcription stimulation is mediated through these factors. Moreover, the two activators differentially recruited the coactivator complexes, consistent with specific activator-coactivator interactions. Chromatinized templates suppressed recruitment of basal transcription factors, thereby amplifying the effect of activators, compared with naked DNA templates. This system is sensitive, highly reproducible, and easily applicable to mapping the repertoire of proteins found at any promoter.

Status: Published Type: Journal Article PubMed ID: 22902623

Topics addressed in this paper

Number of different genes curated to this paper: 111

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Topics Genes linked to topics (#1 - 10 )
ACT1 ADA2 ARP4 ARP5 ARP6 ARP7 ARP8 ARP9 ASH1 BDF1
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Topics Genes linked to topics (#11 - 20 )
CHD1 CTI6 DEP1 DLS1 DOT6 DPB4 EAF1 EAF3 EAF5 EAF6
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Topics Genes linked to topics (#21 - 30 )
EAF7 EPL1 ESA1 GAL4 GCN5 GSP1 HFI1 HIS4 HTL1 IES1
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Topics Genes linked to topics (#31 - 40 )
IES3 IES4 INO80 IOC2 IOC3 IOC4 ISW1 ISW2 ITC1 LDB7
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Topics Genes linked to topics (#41 - 50 )
NGG1 NHP10 NPL6 PHO23 RCO1 RPB3 RPD3 RSC1 RSC2 RSC3
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Topics Genes linked to topics (#51 - 60 )
RSC30 RSC4 RSC58 RSC6 RSC8 RSC9 RTT102 RVB1 RVB2 RXT2
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Topics Genes linked to topics (#61 - 70 )
RXT3 SAP30 SAS2 SAS4 SAS5 SDS3 SFH1 SGF11 SGF29 SGF73
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Topics Genes linked to topics (#71 - 80 )
SIN3 SIR3 SNF11 SNF12 SNF2 SNF5 SNF6 SPT15 SPT16 SPT20
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Topics Genes linked to topics (#81 - 90 )
SPT3 SPT7 SPT8 STH1 SUS1 SWC3 SWC4 SWC5 SWC7 SWI1
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Topics Genes linked to topics (#91 - 100 )
SWI3 SWP82 SWR1 TAF10 TAF12 TAF14 TAF5 TAF6 TAF9 TFA1
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Topics Genes linked to topics (#101 - 110 )
TFB1 TFG2 TOD6 TRA1 UBP8 UME1 UME6 VPS71 VPS72 YAF9
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Topics Genes linked to topics (#111 )
YNG2
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