SGD Paper Help


Page Contents:
Blewett NH and Goldstrohm AC  (2012) A eukaryotic translation initiation factor 4E-binding protein promotes mRNA decapping and is required for PUF repression. Mol Cell Biol 32(20):4181-94

Abstract: PUF proteins are eukaryotic RNA-binding proteins that repress specific mRNAs. The mechanisms and corepressors involved in PUF repression remain to be fully identified. Here, we investigated the mode of repression by Saccharomyces cerevisiae Puf5p and Puf4p and found that Puf5p specifically requires Eap1p to repress mRNAs, whereas Puf4p does not. Surprisingly, we observed that Eap1p, which is a member of the eukaryotic translation initiation factor 4E (eIF4E)-binding protein (4E-BP) class of translational inhibitors, does not inhibit the efficient polyribosome association of a Puf5p target mRNA. Rather, we found that Eap1p accelerates mRNA degradation by promoting decapping, and the ability of Eap1p to interact with eIF4E facilitates this activity. Deletion of EAP1 dramatically reduces decapping, resulting in accumulation of deadenylated, capped mRNA. In support of this phenotype, Eap1p associates both with Puf5p and the Dhh1p decapping factor. Furthermore, recruitment of Eap1p to downregulated mRNA is mediated by Puf5p. On the basis of these results, we propose that Puf5p promotes decapping by recruiting Eap1p and associated decapping factors to mRNAs. The implication of these findings is that a 4E-BP can repress protein expression by promoting specific mRNA degradation steps in addition to or in lieu of inhibiting translation initiation.

Status: Published Type: Journal Article PubMed ID: 22890846

Topics addressed in this paper

Number of different genes curated to this paper: 8

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
CAF20 CDC33 DHH1 EAP1 HO MPT5 PUF4 RNR1
Additional Literature blue ball blue ball blue ball blue ball blue ball
Cellular Location blue ball blue ball
Function/Process blue ball blue ball
Genetic Interactions blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball
Regulation of blue ball
Regulatory Role blue ball blue ball
RNA Levels and Processing blue ball blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: