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Zuzuarregui A, et al.  (2012) M-Track: detecting short-lived protein-protein interactions in vivo. Nat Methods 9(6):594-6

Abstract: We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities among proteins in a mitogen-activated protein kinase pathway and to detect short-lived interactions between protein phosphatase 2A and its substrates that have so far escaped direct detection.

Status: Published Type: Journal Article | Research Support, Non-U.S. Gov't PubMed ID: 22581371

Topics addressed in this paper

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Topics Genes linked to topics (#1 - 10 )
CDC55 FPR1 KIN4 NET1 PBS2 PPH21 RTS1 SHO1 SSK2 SSK22
Additional Literature blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball
Genetic Interactions blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball blue ball blue ball
Protein Processing/Modification/Regulation blue ball blue ball
Protein Sequence Features blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Regulatory Role blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball blue ball

Topics Genes linked to topics (#11 - 12 )
STE11 TOR1
Additional Literature blue ball blue ball
Genetic Interactions blue ball blue ball
Mutants/Phenotypes blue ball blue ball
Strains/Constructs blue ball blue ball

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