Zuzuarregui A, et al. (2012) M-Track: detecting short-lived protein-protein interactions in vivo. Nat Methods 9(6):594-6
Abstract: We developed a protein-proximity assay in yeast based on fusing a histone lysine methyltransferase onto a bait and its substrate onto a prey. Upon binding, the prey is stably methylated and detected by methylation-specific antibodies. We applied this approach to detect varying interaction affinities among proteins in a mitogen-activated protein kinase pathway and to detect short-lived interactions between protein phosphatase 2A and its substrates that have so far escaped direct detection.
|Status: Published||Type: Journal Article | Research Support, Non-U.S. Gov't||PubMed ID: 22581371|
Topics addressed in this paper
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