SGD Paper Help


Page Contents:
Armstrong AA, et al.  (2012) Recognition of SUMO-modified PCNA requires tandem receptor motifs in Srs2. Nature 483(7387):59-63

Abstract: Ubiquitin (Ub) and ubiquitin-like (Ubl) modifiers such as SUMO (also known as Smt3 in Saccharomyces cerevisiae) mediate signal transduction through post-translational modification of substrate proteins in pathways that control differentiation, apoptosis and the cell cycle, and responses to stress such as the DNA damage response. In yeast, the proliferating cell nuclear antigen PCNA (also known as Pol30) is modified by ubiquitin in response to DNA damage and by SUMO during S phase. Whereas Ub-PCNA can signal for recruitment of translesion DNA polymerases, SUMO-PCNA signals for recruitment of the anti-recombinogenic DNA helicase Srs2. It remains unclear how receptors such as Srs2 specifically recognize substrates after conjugation to Ub and Ubls. Here we show, through structural, biochemical and functional studies, that the Srs2 carboxy-terminal domain harbours tandem receptor motifs that interact independently with PCNA and SUMO and that both motifs are required to recognize SUMO-PCNA specifically. The mechanism presented is pertinent to understanding how other receptors specifically recognize Ub- and Ubl-modified substrates to facilitate signal transduction.

Status: Published Type: Journal Article | Research Support, N.I.H., Extramural | Research Support, U.S. Gov't, Non-P.H.S. PubMed ID: 22382979

Topics addressed in this paper

Number of different genes curated to this paper: 6

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
ECO1 POL30 RAD18 RAD6 SMT3 SRS2
Additional Literature blue ball blue ball
Genetic Interactions blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball
Protein Processing/Modification/Regulation blue ball
Protein Sequence Features blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball
Protein/Nucleic Acid Structure blue ball blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: