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Mizuta M, et al.  (2012) Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon. Microbiol Res 167(5):311-6

Abstract: Proteobacterium Escherichia coli strains harboring wide-transfer-range conjugative plasmids are able to transfer these plasmids to several yeast species. Whole plasmid DNA is mobilizable in the transkingdom conjugation phenomenon. Owing to the availability of various conjugative plasmids in bacteria, the horizontal DNA transfer has potential to occur between various bacteria and eukaryotes. In order to know host factor genes involved in such conjugation, we systematically tested the conjugability of strains among a yeast library comprising single-gene-knockout mutants in this study. This genome-wide screen identified 26 host chromosomal genes whose absence reduced the efficiency of the transkingdom conjugation. Among the 26 genes, 20 are responsible for vacuolar ATPase activity, while 5 genes (SHP1, CSG2, CCR4, NOT5, and HOF1) seem to play a role in maintaining the cell surface. Lack of either ZUO1 gene or SSZ1 gene, each of which encodes a component of the ribosome-associated cytoplasmic molecular chaperone, also strongly affected transkingdom conjugation.

Status: Published Type: Journal Article PubMed ID: 22169356

Topics addressed in this paper

Number of different genes curated to this paper: 35

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
CCR4 CSG2 END3 ENT1 ENT2 FAB1 HOF1 INP52 NOT5 PEP3
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Topics Genes linked to topics (#11 - 20 )
SHP1 SSB1 SSZ1 STV1 VMA1 VMA10 VMA11 VMA13 VMA16 VMA2
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Topics Genes linked to topics (#21 - 30 )
VMA21 VMA22 VMA3 VMA4 VMA5 VMA6 VMA7 VMA8 VMA9 VPH1
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Topics Genes linked to topics (#31 - 35 )
VPH2 VPS15 VPS34 YKL118W ZUO1
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