Cho US and Harrison SC (2011) Ndc10 is a platform for inner kinetochore assembly in budding yeast.LID - 10.1038/nsmb.2178 [doi] Nat Struct Mol Biol ()
Abstract: Kinetochores link centromeric DNA to spindle microtubules and ensure faithful chromosome segregation during mitosis. In point-centromere yeasts, the CBF3 complex Skp1-Ctf13-(Cep3)(2)-(Ndc10)(2) recognizes a conserved centromeric DNA element through contacts made by Cep3 and Ndc10. We describe here the five-domain organization of Kluyveromyces lactis Ndc10 and the structure at 2.8 A resolution of domains I-II (residues 1-402) bound to DNA. The structure resembles tyrosine DNA recombinases, although it lacks both endonuclease and ligase activities. Structural and biochemical data demonstrate that each subunit of the Ndc10 dimer binds a separate fragment of DNA, suggesting that Ndc10 stabilizes a DNA loop at the centromere. We describe in vitro association experiments showing that specific domains of Ndc10 interact with each of the known inner-kinetochore proteins or protein complexes in budding yeast. We propose that Ndc10 provides a central platform for inner-kinetochore assembly.
|Status: Epub ahead of print||Type: Journal Article||PubMed ID: 22139014|
Topics addressed in this paper
- To go to the Locus page for a gene, click on the gene name.
|Fungal Related Genes/Proteins|
|Protein/Nucleic Acid Structure|