SGD Paper Help


Page Contents:
Schaefer JB and Morgan DO  (2011) Protein-linked ubiquitin chain structure restricts activity of deubiquitinating enzymes. J Biol Chem 286(52):45186-96

Abstract: The attachment of lysine 48 (Lys(48))-linked polyubiquitin chains to proteins is a universal signal for degradation by the proteasome. Here, we report that long Lys(48)-linked chains are resistant to many deubiquitinating enzymes (DUBs). Representative enzymes from this group, Ubp15 from yeast and its human ortholog USP7, rapidly remove mono- and diubiquitin from substrates but are slow to remove longer Lys(48)-linked chains. This resistance is lost if the structure of Lys(48)-linked chains is disrupted by mutation of ubiquitin or if chains are linked through Lys(63). In contrast to Ubp15 and USP7, Ubp12 readily cleaves the ends of long chains, regardless of chain structure. We propose that the resistance to many DUBs of long, substrate-attached Lys(48)-linked chains helps ensure that proteins are maintained free from ubiquitin until a threshold of ubiquitin ligase activity enables degradation.

Status: Published Type: Journal Article PubMed ID: 22072716

Topics addressed in this paper

Number of different genes curated to this paper: 21

Jump to Summary Chart for:
  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics (#1 - 10 )
DOA4 OTU1 OTU2 PDS1 RPN11 UBP1 UBP10 UBP11 UBP12 UBP13
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Alias blue ball
Primary Literature blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Processing/Modification/Regulation blue ball
Protein Sequence Features blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#11 - 20 )
UBP14 UBP15 UBP16 UBP2 UBP3 UBP5 UBP6 UBP7 UBP8 UBP9
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Non-Fungal Related Genes/Proteins blue ball
Primary Literature blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Sequence Features blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#21 )
YUH1
Additional Literature blue ball
Protein Physical Properties blue ball
Strains/Constructs blue ball
Substrates/Ligands/Cofactors blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: