Lanthaler K, et al. (2011) Genome-wide assessment of the carriers involved in the cellular uptake of drugs: a model system in yeast. BMC Biol 9(1):70
Abstract: ABSTRACT: BACKGROUND: The uptake of drugs into cells has traditionally been considered to be predominantly via passive diffusion through the bilayer portion of the cell membrane. The recent recognition that drug uptake is mostly carrier-mediated, raises the question of which drugs use which carriers. RESULTS: To answer this, we have constructed a chemical genomics platform built upon the yeast gene deletion collection using competition experiments in batch fermenters and robotic automation of cytotoxicity screens, including protection by 'natural' substrates. Using these, we tested 26 different drugs and identified the carriers required for 18 of the drugs to gain entry into yeast cells. CONCLUSIONS: As well as providing a useful platform technology, these results further substantiate the notion that the cellular uptake of pharmaceutical drugs normally occurs via carrier-mediated transport and indicates that establishing the identity and tissue distribution of such carriers should be a major consideration in the design of safe and effective drugs.
|Status: Epub ahead of print||Type: Journal Article||PubMed ID: 22023736|
Topics addressed in this paper
Number of different genes curated to this paper: 19
- To go to the Locus page for a gene, click on the gene name.
|Topics||Topics not linked to Genes||Genes (#1 - 10 )|
|Large-scale phenotype analysis|
|Topics||Genes (#11 - 19 )|