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Boettner DR, et al.  (2011) Clathrin light chain directs endocytosis by influencing the binding of the yeast Hip1R homologue, Sla2, to F-actin. Mol Biol Cell 22(19):3699-714

Abstract: The role of clathrin light chain (CLC) in clathrin-mediated endocytosis is not completely understood. Previous studies showed that the CLC N-terminus (CLC-NT) binds the Hip1/Hip1R/Sla2 family of membrane/actin-binding factors and that overexpression of the CLC-NT in yeast suppresses endocytic defects of clathrin heavy-chain mutants. To elucidate the mechanistic basis for this suppression, we performed synthetic genetic array analysis with a clathrin CLC-NT deletion mutation (clc1-?19-76). clc1-?19-76 suppressed the internalization defects of null mutations in three late endocytic factors: amphiphysins (rvs161 and rvs167) and verprolin (vrp1). In actin sedimentation assays, CLC binding to Sla2 inhibited Sla2 interaction with F-actin. Furthermore, clc1-?19-76 suppression of the rvs and vrp phenotypes required the Sla2 actin-binding talin-Hip1/R/Sla2 actin-tethering C-terminal homology domain, suggesting that clc1-?19-76 promotes internalization by prolonging actin engagement by Sla2. We propose that CLC directs endocytic progression by pruning the Sla2-actin attachments in the clathrin lattice, providing direction for membrane internalization.

Status: Published Type: Journal Article PubMed ID: 21849475

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Number of different genes curated to this paper: 66

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ABP1 ACT1 ALG2 APL4 APM1 APS1 ARL1 ARP3 BER1 BRR1
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Topics Genes linked to topics (#11 - 20 )
CBF1 CEX1 CLC1 COP1 CPR7 CSE2 ECM8 EDE1 ELM1 EMI5
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Topics Genes linked to topics (#21 - 30 )
EST1 FIS1 FMP23 GAP1 GET2 GET3 GET4 GGA1 HOM6 INP53
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Topics Genes linked to topics (#31 - 40 )
LAS17 LSM6 LST8 MAP2 MCD4 MYO3 MYO5 PAN1 PAT1 PEP8
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Topics Genes linked to topics (#41 - 50 )
PEX27 PIK1 PMA2 ROD1 RPL14A RVS161 RVS167 SEC2 SEC21 SHE4
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Topics Genes linked to topics (#51 - 60 )
SIN3 SLA1 SLA2 SNU66 SYS1 UBP6 VAC14 VPH1 VPS21 VPS29
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Topics Genes linked to topics (#61 - 66 )
VPS35 VPS5 VRP1 YIF1 YIP1 YMR166C
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