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Chen X, et al.  (2011) Cell cycle regulation of DNA double-strand break end resection by Cdk1-dependent Dna2 phosphorylation.LID - 10.1038/nsmb.2105 [doi] Nat Struct Mol Biol ()

Abstract: DNA recombination pathways are regulated by the cell cycle to coordinate with replication. Cyclin-dependent kinase (Cdk1) promotes efficient 5' strand resection at DNA double-strand breaks (DSBs), the initial step of homologous recombination and damage checkpoint activation. The Mre11-Rad50-Xrs2 complex with Sae2 initiates resection, whereas two nucleases, Exo1 and Dna2, and the DNA helicase-topoisomerase complex Sgs1-Top3-Rmi1 generate longer ssDNA at DSBs. Using Saccharomyces cerevisiae, we provide evidence for Cdk1-dependent phosphorylation of the resection nuclease Dna2 at Thr4, Ser17 and Ser237 that stimulates its recruitment to DSBs, resection and subsequent Mec1-dependent phosphorylation. Poorly recruited dna2(T4A S17A S237A) and dna2DeltaN248 mutant proteins promote resection only in the presence of Exo1, suggesting cross-talk between Dna2- and Exo1-dependent resection pathways.

Status: Epub ahead of print Type: Journal Article PubMed ID: 21841787

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Topics Genes linked to topics (#1 - 10 )
CDC28 CHK1 DNA2 DUN1 EXO1 MEC1 PIF1 RAD53 SGS1 SML1
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Topics Genes linked to topics (#11 - 12 )
TEL1 YKU70
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Genetic Interactions blue ball
Mutants/Phenotypes blue ball
Regulatory Role blue ball
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