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Arlt H, et al.  (2011) An overexpression screen in Saccharomyces cerevisiae identifies novel genes that affect endocytic protein trafficking. Traffic 12(11):1592-603

Abstract: A large number of proteins involved in the biogenesis of yeast endosomes and vacuoles have been identified based on screens that scored for inactivation of proteins. Such screens may, however, miss important regulators of the pathway. Here, we present a visual screen in which we examined the effects on vacuole morphology if any of the 6153 yeast open reading frames was overexpressed. Using a progressive screening procedure, we could identify a total of 53 genes. Among the most striking endosomal proteins are the CORVET/HOPS subunits Vps3, Vps18 and Vps39 and the putative tethering inhibitor Ivy1. Furthermore, six endosomal sorting complex related to transport (ESCRT) proteins led to altered vacuole morphology if overproduced. Among the novel proteins, we identify Yer128w as an endosomal protein that interacts with the AAA-ATPase Vps4, and therefore named it Vfa1 (Vps Four-Associated 1). We present evidence on the possible role of these novel proteins in trafficking to the vacuole. Our data provide novel insights into the regulation of protein trafficking.

Status: Published Type: Journal Article PubMed ID: 21777356

Topics addressed in this paper

Number of different genes curated to this paper: 53

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
ADY4 ARL1 ATG15 AVT7 BNI4 BRO1 BUL1 CDC34 CLB6 DID4
Large-scale phenotype analysis yg ball
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Omics yg ball
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Topics Genes linked to topics (#11 - 20 )
ECM7 FRK1 GIC1 IFA38 IVY1 PEP12 PEP3 PFA4 PPH3 PRC1
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#21 - 30 )
SAN1 SNA2 SRP21 STB3 STE4 STP22 SWT1 VAM6 VFA1 VPS25
Additional Literature blue ball
Cellular Location blue ball blue ball
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Protein-protein Interactions blue ball
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Topics Genes linked to topics (#31 - 40 )
VPS28 VPS3 VPS4 VTC1 VTH1 YBL094C YBR071W YDL162C YDR514C YDR541C
Cellular Location blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#41 - 50 )
YGL052W YGR204C-A YHC3 YHR112C YIL089W YKL222C YKR041W YOL159C-A YOR223W YOR309C
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Topics Genes linked to topics (#51 - 53 )
YOR314W YPK1 YPT52
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Primary Literature blue ball blue ball blue ball
Strains/Constructs blue ball blue ball blue ball
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