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Sakata E, et al.  (2011) The catalytic activity of Ubp6 enhances maturation of the proteasomal regulatory particle. Mol Cell 42(5):637-49

Abstract: The 26S proteasome is a 2.5 MDa macromolecular machine responsible for targeted protein degradation. Recently, four chaperones were identified that promote the assembly of the 19S regulatory particle (RP). Here, we probe the dynamic architecture of the proteasome by applying quantitative proteomics and mass spectrometry (MS) of intact complexes to provide a detailed characterization of how Ubp6 assists this assembly process. Our MS data demonstrate stoichiometric binding of chaperones and Ubp6 to the basal part of the RP. Genetic interactions of Ubp6 with Hsm3, but not with the other chaperones, indicate a functional overlay with Hsm3. Our biochemical data identified Ubp6 as an additional member of the Hsm3 module. Deletions of ubp6 with hsm3 perturb 26S proteasome assembly, which we attribute to an accumulation of ubiquitylated substrates on these assembly precursors. We therefore propose that Ubp6 facilitates proteasomal assembly by clearing ubiquitylated substrates from assembly precursors by its deubiquitylating activity.CI - Copyright (c) 2011 Elsevier Inc. All rights reserved.

Status: Published Type: Journal Article | Research Support, Non-U.S. Gov't PubMed ID: 21658604

Topics addressed in this paper

Number of different genes curated to this paper: 41

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Topics Genes linked to topics (#1 - 10 )
BLM10 ECM29 HSM3 NAS2 NAS6 PRE1 PRE10 PRE2 PRE3 PRE4
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Cellular Location blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball
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Topics Genes linked to topics (#11 - 20 )
PRE5 PRE6 PRE7 PRE8 PRE9 PUP1 PUP2 PUP3 RPN1 RPN10
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Cellular Location blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#21 - 30 )
RPN11 RPN12 RPN13 RPN14 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Cellular Location blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SCL1 SEM1 UBI4
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Cellular Location blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#41 )
UBP6
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Function/Process blue ball
Genetic Interactions blue ball
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