Koivomagi M, et al. (2011) Dynamics of Cdk1 Substrate Specificity during the Cell Cycle. Mol Cell 42(5):610-23
Abstract: Cdk specificity is determined by the intrinsic selectivity of the active site and by substrate docking sites on the cyclin subunit. There is a long-standing debate about the relative importance of these factors in the timing of Cdk1 substrate phosphorylation. We analyzed major budding yeast cyclins (the G1/S-cyclin Cln2, S-cyclin Clb5, G2/M-cyclin Clb3, and M-cyclin Clb2) and found that the activity of Cdk1 toward the consensus motif increased gradually in the sequence Cln2-Clb5-Clb3-Clb2, in parallel with cell cycle progression. Further, we identified a docking element that compensates for the weak intrinsic specificity of Cln2 toward G1-specific targets. In addition, Cln2-Cdk1 showed distinct consensus site specificity, suggesting that cyclins do not merely activate Cdk1 but also modulate its active-site specificity. Finally, we identified several Cln2-, Clb3-, and Clb2-specific Cdk1 targets. We propose that robust timing and ordering of cell cycle events depend on gradual changes in the substrate specificity of Cdk1.CI - Copyright (c) 2011 Elsevier Inc. All rights reserved.
|Status: Published||Type: Journal Article||PubMed ID: 21658602|
Topics addressed in this paper
Number of different genes curated to this paper: 14
- To go to the Locus page for a gene, click on the gene name.
|Topics||Genes (#1 - 10 )|
|Protein Physical Properties|
|Protein Sequence Features|
|Topics||Genes (#11 - 14 )|