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Benjamin JJ, et al.  (2011) Dysregulated Arl1, a regulator of post-Golgi vesicle tethering, can inhibit endosomal transport and cell proliferation in yeast. Mol Biol Cell 22(13):2337-47

Abstract: Small monomeric G proteins regulated in part by GTPase-activating proteins (GAPs) are molecular switches for several aspects of vesicular transport. The yeast Gcs1 protein is a dual-specificity GAP for ADP-ribosylation factor (Arf) and Arf-like (Arl)1 G proteins, and also has GAP-independent activities. The absence of Gcs1 imposes cold sensitivity for growth and endosomal transport; here we present evidence that dysregulated Arl1 may cause these impairments. We show that gene deletions affecting the Arl1 or Ypt6 vesicle-tethering pathways prevent Arl1 activation and membrane localization, and restore growth and trafficking in the absence of Gcs1. A mutant version of Gcs1 deficient for both ArfGAP and Arl1GAP activity in vitro still allows growth and endosomal transport, suggesting that the function of Gcs1 that is required for these processes is independent of GAP activity. We propose that, in the absence of this GAP-independent regulation by Gcs1, the resulting dysregulated Arl1 prevents growth and impairs endosomal transport at low temperatures. In cells with dysregulated Arl1, an increased abundance of the Arl1 effector Imh1 restores growth and trafficking, and does so through Arl1 binding. Protein sequestration at the trans-Golgi membrane by dysregulated, active Arl1 may therefore be the mechanism of inhibition.

Status: Published Type: Journal Article PubMed ID: 21562219

Topics addressed in this paper

Number of different genes curated to this paper: 54

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Topics Genes linked to topics (#1 - 10 )
AAT2 AGE2 AKR1 ARF1 ARL1 ARL3 AVT6 BEM2 CAN1 CAX4
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Topics Genes linked to topics (#11 - 20 )
CBF1 CHD1 CHL1 CKI1 EGD1 EST1 FAA3 GCS1 HXT8 IMH1
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Topics Genes linked to topics (#21 - 30 )
LST7 LYP1 MAK10 MAK3 MAK31 MDY2 MET32 MNN9 MTC5 MUS81
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Topics Genes linked to topics (#31 - 40 )
NAT1 NPL6 OAF1 PDX3 PER1 PET18 POP2 POS5 PPH21 PTC1
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Topics Genes linked to topics (#41 - 50 )
RGP1 RIC1 RPE1 RPL24A STP1 SYS1 VPS61 VPS63 YCK2 YCK3
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Topics Genes linked to topics (#51 - 54 )
YPR050C YPT31 YPT32 YPT6
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