Kim KY and Levin DE (2011) Mpk1 MAPK association with the paf1 complex blocks sen1-mediated premature transcription termination. Cell 144(5):745-56
Abstract: The Mpk1 MAPK of the yeast cell wall integrity pathway uses a noncatalytic mechanism to activate transcription of stress-induced genes by recruitment of initiation factors to target promoters. We show here that Mpk1 additionally serves a function in transcription elongation that is also independent of its catalytic activity. This function is mediated by an interaction between Mpk1 and the Paf1 subunit of the Paf1C elongation complex. A mutation in Paf1 that blocks this interaction causes a specific defect in transcription elongation of an Mpk1-induced gene, which results from Sen1-dependent premature termination through a Nab3-binding site within the promoter-proximal region of the gene. Our findings reveal a regulatory mechanism in which Mpk1 overcomes transcriptional attenuation by blocking recruitment of the Sen1-Nrd1-Nab3 termination complex to the elongating polymerase. Finally, we demonstrate that this mechanism is conserved in an interaction between the human ERK5 MAPK and human Paf1.CI - Copyright (c) 2011 Elsevier Inc. All rights reserved.
|Status: Published||Type: Journal Article||PubMed ID: 21376235|
Topics addressed in this paper
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|Topics||Genes linked to topics (#1 - 10 )|
|Non-Fungal Related Genes/Proteins|
|Topics||Genes linked to topics (#11 - 12 )|
|Protein-Nucleic Acid Interactions|