Song W, et al. (2011) Candida albicans Sfl2, a temperature-induced transcriptional regulator, is required for virulence in a murine gastrointestinal infection model. FEMS Yeast Res 11(2):209-22
Abstract: Morphological transition between yeast and filamentous forms of Candida albicans is controlled by complex regulatory machinery that involves many transcriptional regulators playing positive or negative roles. Recently, Sfl2, a sequence homolog of C. albicans Sfl1, has been shown to be required for hyphal development. In this report, we show that, like C. albicans Sfl1, Sfl2 could complement the phenotypes of the Saccharomyces cerevisiae sfl1 mutant, and GFP tagged Sfl2 localized in the nuclei of both yeast and hyphal cells in C. albicans, reflecting its role as a transcriptional regulator. In C. albicans, SFL2 expression was induced at high growth temperature (37 degrees C) at both transcriptional and translational levels. Deletion of SFL2 impaired filamentation at high temperature, whereas overexpression of SFL2 promoted filamentous growth at low temperature. Sfl2-activated hyphal development needs the existence of Efg1 and Flo8 in aerobic conditions. Thus, in contrast to Sfl1 which represses filamentation, Sfl2 acts as an activator of filamentous growth in C. albicans. Functional analysis of chimeric Sfl proteins demonstrated that the opposite actions of C. albicans Sfl1 and Sfl2 were mainly mediated by their HSF domains. Furthermore, deletion of SFL2 attenuated virulence in a mouse model of gastrointestinal colonization and dissemination, indicating that Sfl2 is important for virulence in the GI model of candidiasis. Our results provide new insights into Sfl2 functions in C. albicans morphogenesis and pathogenesis.CI - (c) 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
|Status: Published||Type: Journal Article||PubMed ID: 21205158|
Topics addressed in this paper
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|Fungal Related Genes/Proteins|