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Reid RJ, et al.  (2011) Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage. Genome Res 21(3):477-86

Abstract: We have streamlined the process of transferring plasmids into any yeast strain library by developing a novel mating-based, high-throughput method called selective ploidy ablation (SPA). SPA uses a universal plasmid donor strain that contains conditional centromeres on every chromosome. The plasmid-bearing donor is mated to a recipient followed by removal of all donor strain chromosomes, producing a haploid strain containing the transferred plasmid. As proof of principle, we used SPA to transfer plasmids containing wild-type and mutant alleles of DNA topoisomerase I (TOP1) into the haploid yeast gene disruption library. Over-expression of Top1 identified only one sensitive mutation, rpa34, while over-expression of top1-T722A allele, a camptothecin mimetic, identified 190 sensitive gene disruption strains along with rpa34. In addition to known camptothecin sensitive strains, this set contained mutations in genes involved in the Rpd3 histone deacetylase complex, the kinetochore and vesicle trafficking. We further show that mutations in several ESCRT vesicle trafficking components increase Top1 levels, which is dependent on SUMO modification. These findings demonstrate the utility of the SPA technique to introduce plasmids into the haploid gene disruption library to discover new interacting pathways.

Status: Published Type: Journal Article PubMed ID: 21173034

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Number of different genes curated to this paper: 220

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
APS1 ARN1 ARO10 ASF1 ATP14 BRO1 BRP1 BSD2 BUD31 CCH1
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Topics Genes linked to topics (#11 - 20 )
CCR4 CDC50 CHL4 CIN5 CLB2 CLB5 CLN3 COQ10 CPA1 CPR1
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Topics Genes linked to topics (#21 - 30 )
CSM3 CTF18 CTF19 CTF4 CTF8 DCC1 DDC1 DEP1 DIA2 DID2
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Topics Genes linked to topics (#31 - 40 )
DID4 DOA4 DOC1 DUS4 EAF1 EMC2 FAA1 FAB1 FRE4 FTR1
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Topics Genes linked to topics (#41 - 50 )
FUN30 FUR4 FYV4 GET2 GGC1 GLO3 GRE2 GZF3 HFD1 HMG2
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Topics Genes linked to topics (#51 - 60 )
HSP31 HTD2 HUR1 IMH1 IRC15 LSM6 LTE1 MCM16 MCM21 MCM22
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Topics Genes linked to topics (#61 - 70 )
MDV1 MEC3 MET7 MMS1 MMS2 MMS4 MRE11 MRPL11 MRPL22 MRPL28
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Topics Genes linked to topics (#71 - 80 )
MRPS9 MSH4 MSL1 MUS81 MVB12 NCA3 NHX1 NPT1 NSR1 NTG2
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Topics Genes linked to topics (#81 - 90 )
NUP133 NUP60 NUP84 OCA1 OMA1 PAT1 PEP8 PEX5 PFS1 PHO23
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Topics Genes linked to topics (#91 - 100 )
PHO84 PKR1 PMP1 PMP3 PMR1 POP2 POR1 PPH3 PRM10 PRY2
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Topics Genes linked to topics (#101 - 110 )
PSD2 PSY2 PTC1 QCR10 RAD17 RAD24 RAD27 RAD50 RAD51 RAD52
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Topics Genes linked to topics (#111 - 120 )
RAD54 RAD55 RAD57 RAD59 RAD61 RAD9 RAS2 RAV2 RGP1 RIC1
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Topics Genes linked to topics (#121 - 130 )
RMI1 RNP1 RNR4 RPA34 RPD3 RPL13B RPL20B RRF1 RRM3 RRT8
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Topics Genes linked to topics (#131 - 140 )
RSM25 RSM7 RTT101 RTT107 RTT109 RUB1 RVS167 SAC6 SAE2 SAM37
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Topics Genes linked to topics (#141 - 150 )
SAP30 SHE4 SIN3 SIS2 SKI8 SKT5 SLA1 SLX4 SNF7 SNF8
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Topics Genes linked to topics (#151 - 160 )
SOD1 SPT2 SPT21 SRC1 SRN2 SRS2 SSZ1 STE50 STL1 STP22
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Topics Genes linked to topics (#161 - 170 )
TDA1 TDA10 TDA11 TDA2 TDA3 TDA4 TDA5 TDA6 TDA7 TDA8
Genetic Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#171 - 180 )
TDA9 TDP1 TEL1 TGL3 TNA1 TOF1 TOP1 TOP3 UGO1 URA5
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Primary Literature blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#181 - 190 )
VAM10 VAM3 VAM6 VMA13 VMA5 VPS1 VPS13 VPS17 VPS20 VPS21
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#191 - 200 )
VPS24 VPS25 VPS27 VPS28 VPS29 VPS35 VPS36 VPS38 VPS4 VPS5
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#201 - 210 )
VPS60 VPS8 VPS9 VTA1 XRS2 YBP1 YBR099C YBR224W YDR455C YGL214W
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#211 - 220 )
YGR160W YHR127W YHR131C YHR180W YLR169W YLR235C YLR294C YML122C YPT6 ZUO1
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