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Mitchell SF, et al.  (2010) The 5'-7-Methylguanosine Cap on Eukaryotic mRNAs Serves Both to Stimulate Canonical Translation Initiation and to Block an Alternative Pathway. Mol Cell 39(6):950-62

Abstract: Translational control is frequently exerted at the stage of mRNA recruitment to the initiating ribosome. We have reconstituted mRNA recruitment to the 43S preinitiation complex (PIC) using purified S. cerevisiae components. We show that eIF3 and the eIF4 factors not only stabilize binding of mRNA to the PIC, they also dramatically increase the rate of recruitment. Although capped mRNAs require eIF3 and the eIF4 factors for efficient recruitment to the PIC, uncapped mRNAs can be recruited in the presence of eIF3 alone. The cap strongly inhibits this alternative recruitment pathway, imposing a requirement for the eIF4 factors for rapid and stable binding of natural mRNA. Our data suggest that the 5' cap serves as both a positive and negative element in mRNA recruitment, promoting initiation in the presence of the canonical group of mRNA handling factors while preventing binding to the ribosome via an aberrant, alternative pathway requiring only eIF3.CI - Copyright (c) 2010 Elsevier Inc. All rights reserved.

Status: Published Type: Journal Article PubMed ID: 20864040

Topics addressed in this paper

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Topics Genes linked to topics (#1 - 10 )
CDC33 DAD4 HCR1 NIP1 PRT1 RPG1 RPL41A SUI1 TIF1 TIF11
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#11 - 16 )
TIF2 TIF34 TIF35 TIF4631 TIF4632 TIF5
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball
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