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Brooks MA, et al.  (2010) Systematic Bioinformatics and Experimental Validation of Yeast Complexes Reduces the Rate of Attrition during Structural Investigations. Structure 18(9):1075-82

Abstract: For high-throughput structural studies of protein complexes of composition inferred from proteomics data, it is crucial that candidate complexes are selected accurately. Herein, we exemplify a procedure that combines a bioinformatics tool for complex selection with in vivo validation, to deliver structural results in a medium-throughout manner. We have selected a set of 20 yeast complexes, which were predicted to be feasible by either an automated bioinformatics algorithm, by manual inspection of primary data, or by literature searches. These complexes were validated with two straightforward and efficient biochemical assays, and heterologous expression technologies of complex components were then used to produce the complexes to assess their feasibility experimentally. Approximately one-half of the selected complexes were useful for structural studies, and we detail one particular success story. Our results underscore the importance of accurate target selection and validation in avoiding transient, unstable, or simply nonexistent complexes from the outset.CI - Copyright (c) 2010 Elsevier Ltd. All rights reserved.

Status: Published Type: Journal Article PubMed ID: 20826334

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ATG17 ATG20 ATG29 CAB3 DCD1 DOP1 DSN1 DUG2 DUG3 EDC3
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Computational analysis yg ball
Omics yg ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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EDE1 GCD10 GCD14 GIR2 HSE1 MEC3 MET12 MET13 MON2 MTW1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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NNF1 NSL1 NSP1 NUP159 NUP82 ORC1 ORC2 ORC3 ORC4 ORC5
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Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
ORC6 PAA1 PPH3 PSY2 PSY4 PTC2 RAD17 RBG2 RPS28B SIS2
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#41 - 49 )
SPN1 SPT6 SSL2 STE11 STE50 SYP1 TFB6 VHS3 VPS27
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Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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