Ren J, et al. (2010) Promoter Strength Influences the S Phase Requirement for Establishment of Silencing at the Saccharomyces cerevisiae Silent Mating Type Loci. Genetics 186(2):551-60
Abstract: In Saccharomyces cerevisiae, the two cryptic mating type loci, HML and HMR, are transcriptionally silent. Previous studies on the establishment of silencing at HMR identified a requirement for passage through S phase. However, the underlying mechanism for this requirement is still unknown. In contrast to HMR, we found that substantial silencing of HML could be established without passage through S-phase. In order to understand this difference, we analyzed several chimeric HM loci and found that promoter strength determined the S phase requirement. To silence a locus with a strong promoter such as the a1/a2 promoter required passage through S phase while HM loci with weaker promoters such as the alpha1/alpha2 or TRP1 promoter did not show this requirement. Thus, transcriptional activity counteracts the establishment of silencing but can be overcome by passage through S phase.
|Status: Published||Type: Journal Article||PubMed ID: 20679515|
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