Flemming D, et al. (2010) Precise mapping of subunits in multiprotein complexes by a versatile electron microscopy label. Nat Struct Mol Biol 17(6):775-8
Abstract: Positional knowledge of subunits within multiprotein assemblies is crucial for understanding their function. The topological analysis of protein complexes by electron microscopy has undergone impressive development, but analysis of the exact positioning of single subunits has lagged behind. Here we have developed a clonable approximately 80-residue tag that, upon attachment to a target protein, can recruit a structurally prominent electron microscopy label in vitro. This tag is readily visible on single particles and becomes exceptionally distinct after image processing and classification. Thus, our method is applicable for the exact topological mapping of subunits in macromolecular complexes.
|Status: Published||Type: Journal Article | Research Support, Non-U.S. Gov't||PubMed ID: 20512149|
Topics addressed in this paper
Number of different genes curated to this paper: 8
- To find other papers on a gene and topic, click on the colored ball in the appropriate box.
- displays other papers with information about that topic for that gene.
- displays other papers in SGD that are associated with that topic.
The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
- To go to the Locus page for a gene, click on the gene name.
|Topics||Topics not linked to Genes||Genes linked to topics|
|Protein Physical Properties|
|Protein Sequence Features|
|Protein/Nucleic Acid Structure|
|Techniques and Reagents|