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Lee SK, et al.  (2010) Activation of a Poised RNAPII-Dependent Promoter Requires Both SAGA and Mediator. Genetics 184(3):659-72

Abstract: A growing number of promoters have key components of the transcription machinery, like TATA-Binding Protein (TBP) and RNA polymerase II (RNAPII), present at the promoter prior to activation of transcription. Thus, while transcriptional output undergoes a dramatic increase between uninduced and induced conditions, occupancy of a large portion of the transcription machinery does not. As such, activation of these poised promoters depends on rate-limiting steps after recruitment of TBP and RNAPII for regulated expression. Little is known about the transcription components required in these latter steps of transcription in vivo. To identify transcription components with critical roles in transcription after recruitment of TBP in Saccharomyces cerevisiae, we screened for loss of gene expression activity from promoter-tethered TBP in over 100 mutant strains deleted for a transcription-related gene. The assay revealed a dramatic enrichment for strains containing deletions in genes encoding subunits of the SAGA complex and Mediator. Analysis of an authentic postrecruitment regulated gene (CYC1) reveals that SAGA occupies the promoter under both uninduced and induced conditions. In contrast, Mediator is recruited only after transfer to inducing conditions, and correlates with activation of the preloaded polymerase at CYC1. These studies indicate critical functions of SAGA and Mediator in the mechanism of activation of genes with rate-limiting steps after recruitment of TBP.

Status: Published Type: Journal Article PubMed ID: 20048049

Topics addressed in this paper

Number of different genes curated to this paper: 104

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Topics Genes linked to topics (#1 - 10 )
ADA2 AHC1 ASC1 BAS1 CAF16 CAF4 CCR4 CDC73 CHD1 CKA1
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Topics Genes linked to topics (#11 - 20 )
CKA2 CKB1 CKB2 CSE2 CTK1 CTK2 CTK3 CYC1 CYC8 DST1
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DNA/RNA Sequence Features blue ball
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Topics Genes linked to topics (#21 - 30 )
EAF3 EAF6 ELA1 ELC1 ELP2 ELP3 ELP4 ELP6 GAL11 GAL4
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Topics Genes linked to topics (#31 - 40 )
GAL80 GCN5 HAT1 HAT2 HDA1 HFI1 HIR2 HOS1 HOS2 HOS3
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Topics Genes linked to topics (#41 - 50 )
HPC2 HST1 IBA57 IKI3 ISW1 ITC1 MBF1 MCM22 MED1 MET18
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Topics Genes linked to topics (#51 - 60 )
MHR1 MOT2 MOT3 NGG1 NHP6A NOT3 NOT5 NRG2 NUT1 PAF1
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Topics Genes linked to topics (#61 - 70 )
PGD1 PHO23 POP2 RAD26 ROX3 RPB4 RPB9 RPD3 RTF1 SAP30
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Topics Genes linked to topics (#71 - 80 )
SGF11 SIN3 SIN4 SNF11 SNF2 SNF5 SNF6 SOH1 SPT10 SPT15
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Alias blue ball
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Protein-Nucleic Acid Interactions blue ball blue ball blue ball
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Topics Genes linked to topics (#81 - 90 )
SPT2 SPT20 SPT21 SPT23 SPT3 SPT4 SPT7 SPT8 SRB2 SRB5
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Topics Genes linked to topics (#91 - 100 )
SRB8 SSN2 SSN3 SSN8 SUT1 SWI3 SWI5 SWI6 TAF14 TBS1
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Topics Genes linked to topics (#101 - 104 )
THP1 THP2 TUP1 YNG1
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