Thomson E and Tollervey D (2010) The final step in 5.8S rRNA processing is cytoplasmic in Saccharomyces cerevisiae. Mol Cell Biol 30(4):976-84
Abstract: The 18S rRNA component of yeast 40S ribosomes undergoes cytoplasmic 3'-cleavage following nuclear export, whereas exported pre-60S subunits were believed to contain only mature 5.8S and 25S rRNAs. However, in situ hybridization detected 3'-extended forms of 5.8S rRNA in the cytoplasm, which were lost when Crm1-dependent pre-ribosome export was blocked by treatment with leptomycin B (LMB). LMB treatment rapidly blocked processing of 6S pre-rRNA to 5.8S rRNA, leading to TRAMP-dependent pre-rRNA degradation. The 6S pre-rRNA was co-precipitated with the 60S export adapter Nmd3 and cytoplasmic 60S synthesis factor Lsg1. The longer 5.8S+30 pre-rRNA is processed to 6S by the nuclear exonuclease Rrp6, and nuclear pre-rRNA accumulated in the absence of Rrp6. In contrast, 6S to 5.8S processing requires the cytoplasmic exonuclease Ngl2, and cytoplasmic pre-rRNA accumulated in strains lacking Ngl2. We conclude that nuclear pre-60S particles containing the 6S pre-rRNA bind Nmd3 and Crm1, and are exported to the cytoplasm prior to final maturation by Ngl2.
|Status: Published||Type: Journal Article||PubMed ID: 20008552|
Topics addressed in this paper
Number of different genes curated to this paper: 11
- To go to the Locus page for a gene, click on the gene name.
|Topics||Genes (#1 - 10 )|
|Protein Sequence Features|
|Protein-Nucleic Acid Interactions|
|RNA Levels and Processing|
|Topics||Genes (#11 )|