SGD Paper 
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Neale MJ and Keeney S (2009) End-labeling and analysis of Spo11-oligonucleotide complexes in Saccharomyces cerevisiae. Methods Mol Biol 557:183-95
Abstract: During meiosis Spo11 catalyzes the formation of DNA double-strand breaks, becoming covalently attached to the 5' ends on both sides of the break during this process. Spo11 is removed from the DSB by single-stranded endonucleolytic cleavage flanking the DSB, liberating a short-lived species consisting of Spo11 protein covalently linked to a short oligonucleotide. The method presented here details how to detect these Spo11-oligo complexes in extracts made from meiotic yeast cells.
| Status: Published | Type: Journal Article | PubMed ID: 19799183 |
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| Topics | Genes linked to topics |
|---|---|
| SPO11 | |
| Additional Literature |  |
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