SGD Paper Help


Page Contents:
Sinha M, et al.  (2009) Recombinational repair within heterochromatin requires ATP-dependent chromatin remodeling. Cell 138(6):1109-21

Abstract: Heterochromatin plays a key role in protection of chromosome integrity by suppressing homologous recombination. In Saccharomyces cerevisiae, Sir2p, Sir3p, and Sir4p are structural components of heterochromatin found at telomeres and the silent mating-type loci. Here we have investigated whether incorporation of Sir proteins into minichromosomes regulates early steps of recombinational repair in vitro. We find that addition of Sir3p to a nucleosomal substrate is sufficient to eliminate yRad51p-catalyzed formation of joints, and that this repression is enhanced by Sir2p/Sir4p. Importantly, Sir-mediated repression requires histone residues that are critical for silencing in vivo. Moreover, we demonstrate that the SWI/SNF chromatin-remodeling enzyme facilitates joint formation by evicting Sir3p, thereby promoting subsequent Rad54p-dependent formation of a strand invasion product. These results suggest that recombinational repair in the context of heterochromatin presents additional constraints that can be overcome by ATP-dependent chromatin-remodeling enzymes.

Status: Published Type: Journal Article | Research Support, N.I.H., Extramural | Research Support, Non-U.S. Gov't PubMed ID: 19766565

Topics addressed in this paper

Number of different genes curated to this paper: 56

Jump to Summary Chart for:
  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics (#1 - 10 )
ACT1 ARP4 ARP5 ARP6 ARP7 ARP8 ARP9 EAF1 HHF1 HHF2
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball
Mutants/Phenotypes blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball
Protein Sequence Features blue ball blue ball
Regulatory Role blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#11 - 20 )
HHT1 HHT2 HTL1 IES1 IES3 IES4 INO80 LDB7 MOT1 NHP10
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball
Regulatory Role blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#21 - 30 )
NPL6 RAD54 RSC1 RSC2 RSC3 RSC30 RSC4 RSC58 RSC6 RSC8
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Regulatory Role blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Jump to Summary Chart for:
  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.

Topics Genes linked to topics (#31 - 40 )
RSC9 RTT102 RVB1 RVB2 SFH1 SIR2 SIR3 SIR4 SNF11 SNF12
Additional Literature blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball blue ball blue ball
Protein Sequence Features blue ball
Protein-Nucleic Acid Interactions blue ball
Protein-protein Interactions blue ball
Regulatory Role blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#41 - 50 )
SNF2 SNF5 SNF6 STH1 SWC3 SWC4 SWC5 SWC7 SWI1 SWI3
Additional Literature blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball
Primary Literature blue ball blue ball blue ball blue ball blue ball
Regulatory Role blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball
Substrates/Ligands/Cofactors blue ball blue ball blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#51 - 56 )
SWP82 SWR1 TAF14 VPS71 VPS72 YAF9
Additional Literature blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball
Primary Literature blue ball blue ball
Regulatory Role blue ball blue ball blue ball blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball
Jump to Summary Chart for:
  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: