Yamamoto K, et al. (2008) Crystallization and preliminary X-ray analysis of isomaltase from Saccharomyces cerevisiae. Acta Crystallogr Sect F Struct Biol Cryst Commun 64(Pt 11):1024-6
Abstract: Isomaltase from Saccharomyces cerevisiae is an oligo-1,6-glucosidase that preferentially hydrolyzes isomaltose, with little activity towards isomaltotriose or longer oligosaccharides. An amino-acid sequence analysis of the isomaltase revealed that it belongs to glucoside hydrolase family 13. Recombinant isomaltase was purified and crystallized by the hanging-drop vapour-diffusion method with PEG 3350 as the precipitant. The crystals belonged to space group C2, with unit-cell parameters a = 95.67, b = 115.42, c = 61.77 A, beta = 91.17 degrees. X-ray diffraction data were collected to 1.35 A resolution from a single crystal on a synchrotron-radiation source.
|Status: Published||Type: Journal Article||PubMed ID: 18997332|
Topics addressed in this paper
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|Protein/Nucleic Acid Structure|