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Hacker I, et al.  (2008) Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy. Nat Struct Mol Biol 15(11):1206-12

Abstract: The U4/U6-U5 tri-small nuclear ribonucleoprotein (snRNP) is a major, evolutionarily highly conserved spliceosome subunit. Unwinding of its U4/U6 snRNA duplex is a central event of spliceosome activation that requires several components of the U5 portion of the tri-snRNP, including the RNA helicase Brr2, Prp8 and the GTPase Snu114. Here we report the EM projection structure of the Saccharomyces cerevisiae tri-snRNP. It shows a modular organization comprising three extruding domains that contact one another in its central portion. We have visualized genetically tagged tri-snRNP proteins by EM and show here that U4/U6 snRNP forms a domain termed the arm. Conversely, a separate head domain adjacent to the arm harbors Brr2, whereas Prp8 and the GTPase Snu114 are located centrally. The head and arm adopt variable relative positions. This molecular organization and dynamics suggest possible scenarios for structural events during catalytic activation.

Status: Published Type: Journal Article PubMed ID: 18953335

Topics addressed in this paper

Number of different genes curated to this paper: 22

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Topics Genes linked to topics (#1 - 10 )
BRR2 DIB1 LIN1 LSM4 LSM8 PRP3 PRP31 PRP38 PRP4 PRP6
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Topics Genes linked to topics (#11 - 20 )
PRP8 SMB1 SMD1 SNR14 SNR6 SNR7-L SNR7-S SNU114 SNU13 SNU23
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Topics Genes linked to topics (#21 - 22 )
SNU66 SPP381
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Protein-protein Interactions blue ball blue ball
Protein/Nucleic Acid Structure blue ball blue ball

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