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Wang W, et al.  (2008) Maternal phosphatase inhibitor-2 is required for proper chromosome segregation and mitotic synchrony during Drosophila embryogenesis. Genetics 179(4):1823-33

Abstract: Protein phosphatase-1 (PP1) is a major Ser/Thr phosphatase conserved among all eukaryotes, present as the essential GLC7 gene in yeast. Inhibitor-2 (I-2) is an ancient PP1 regulator, named GLC8 in yeast, but its in vivo function is unknown. Unlike mammals with multiple I-2 genes, in Drosophila there is a single I-2 gene, and here we describe its maternally-derived expression and required function during embryogenesis. During oogenesis, germline expression of I-2 results in the accumulation of the RNA and abundant protein in unfertilized eggs; in embryos the endogenous I-2 protein concentrates around condensed chromosomes during mitosis and also surrounds interphase nuclei. An I-2 loss-of-function genotype is associated with a maternal effect phenotype that results in drastically reduced progeny viability, as measured by reduced embryonic hatch rates and larval lethality. Embryos derived from I-2 mutant mothers show faulty chromosome segregation and loss of mitotic synchrony in cleavage stage embryos, patchy loss of nuclei in syncytial blastoderms, and cuticular pattern defects in late stage embryos. Transgenic expression of wild-type I-2 in mutant mothers gives dose-dependent rescue of the maternal effect on embryo hatch rate. We propose that I-2 is required for proper chromosome segregation during Drosophila embryogenesis through the coordinated regulation of PP1 and Aurora B.

Status: Published

Type: Journal Article

PubMed ID: 18689877

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Wang W Cronmiller C Brautigan D Papers in SGD Colleagues in SGD PubMed Google Scholar

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