He Q, et al. (2008) Dispersed mutations in histone H3 that affect transcriptional repression and chromatin structure of the CHA1 promoter in Saccharomyces cerevisiae. Eukaryot Cell 7(10):1649-60
Abstract: The histone H3 amino terminus, but not that of H4, is required to prevent the constitutively bound activator Cha4 from remodeling chromatin and activating transcription at the CHA1 gene in yeast. Here we show that neither the modifiable lysine residues nor any specific region of the H3 tail is required for repression of CHA1. We then screened for histone H3 mutations that cause derepression of the uninduced CHA1 promoter and identified six mutants, three of which are also temperature sensitive mutants and four of which exhibit a sin- phenotype. Histone mutant levels were similar to that of wild type H3, and the mutations do not cause gross alterations in nucleosome structure. One specific and strongly derepressing mutation, H3 A111G, was examined in depth and found to cause a constitutively active chromatin configuration at the uninduced CHA1 promoter, as well as at the ADH2 promoter. Transcriptional derepression and altered chromatin structure of the CHA1 promoter depend on the activator Cha4. These results indicate that modest perturbations in distinct regions of the nucleosome can substantially affect the repressive function of chromatin, allowing activation in the absence of normal inducing signal (at CHA1) or of Swi/Snf (resulting in a sin- phenotype).
|Status: Published||Type: Journal Article||PubMed ID: 18658255|
Topics addressed in this paper
Number of different genes curated to this paper: 5
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