---

Klionsky DJ, et al.  (2008) Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 4(2):151-75

Abstract: Research in autophagy continues to accelerate,(1) and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.

Status: Published

Type: Journal Article

PubMed ID: 18188003

---

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:

1. (1) Choose an author,
2. (2) Choose a search parameter,
3. (3) Click to implement

Klionsky DJ Abeliovich H Agostinis P Agrawal DK Aliev G Askew DS Baba M Baehrecke EH Bahr BA Ballabio A Bamber BA Bassham DC Bergamini E Bi X Biard-Piechaczyk M Blum JS Bredesen DE Brodsky JL Brumell JH Brunk UT Bursch W Camougrand N Cebollero E Cecconi F Chen Y Chin LS Choi A Chu CT Chung J Clarke PG Clark RS Clarke SG Clave C Cleveland JL Codogno P Colombo MI Coto-Montes A Cregg JM Cuervo AM Debnath J Demarchi F Dennis PB Dennis PA Deretic V Devenish RJ Di Sano F Dice JF Difiglia M Dinesh-Kumar S Distelhorst CW Djavaheri-Mergny M Dorsey FC Droge W Dron M Dunn WA Jr Duszenko M Eissa NT Elazar Z Esclatine A Eskelinen EL Fesus L Finley KD Fuentes JM Fueyo J Fujisaki K Galliot B Gao FB Gewirtz DA Gibson SB Gohla A Goldberg AL Gonzalez R Gonzalez-Estevez C Gorski S Gottlieb RA Haussinger D He YW Heidenreich K Hill JA Hoyer-Hansen M Hu X Huang WP Iwasaki A Jaattela M Jackson WT Jiang X Jin S Johansen T Jung JU Kadowaki M Kang C Kelekar A Kessel DH Kiel JA Kim HP Kimchi A Kinsella TJ Kiselyov K Kitamoto K Knecht E Komatsu M Kominami E Kondo S Kovacs AL Kroemer G Kuan CY Kumar R Kundu M Landry J Laporte M Le W Lei HY Lenardo MJ Levine B Lieberman A Lim KL Lin FC Liou W Liu LF Lopez-Berestein G Lopez-Otin C Lu B Macleod KF Malorni W Martinet W Matsuoka K Mautner J Meijer AJ Melendez A Michels P Miotto G Mistiaen WP Mizushima N Mograbi B Monastyrska I Moore MN Moreira PI Moriyasu Y Motyl T Munz C Murphy LO Naqvi NI Neufeld TP Nishino I Nixon RA Noda T Nurnberg B Ogawa M Oleinick NL Olsen LJ Ozpolat B Paglin S Palmer GE Papassideri I Parkes M Perlmutter DH Perry G Piacentini M Pinkas-Kramarski R Prescott M Proikas-Cezanne T Raben N Rami A Reggiori F Rohrer B Rubinsztein DC Ryan KM Sadoshima J Sakagami H Sakai Y Sandri M Sasakawa C Sass M Schneider C Seglen PO Seleverstov O Settleman J Shacka JJ Shapiro IM Sibirny A Silva-Zacarin EC Simon HU Simone C Simonsen A Smith MA Spanel-Borowski K Srinivas V Steeves M Stenmark H Stromhaug PE Subauste CS Sugimoto S Sulzer D Suzuki T Swanson MS Tabas I Takeshita F Talbot NJ Talloczy Z Tanaka K Tanaka K Tanida I Taylor GS Taylor JP Terman A Tettamanti G Thompson CB Thumm M Tolkovsky AM Tooze SA Truant R Tumanovska LV Uchiyama Y Ueno T Uzcategui NL van der Klei I Vaquero EC Vellai T Vogel MW Wang HG Webster P Wiley JW Xi Z Xiao G Yahalom J Yang JM Yap G Yin XM Yoshimori T Yu L Yue Z Yuzaki M Zabirnyk O Zheng X Zhu X Deter RL Papers in SGD Colleagues in SGD PubMed Google Scholar

---