SGD Paper 
Takahashi S and Pryciak PM (2007) Identification of Novel Membrane-binding Domains in Multiple Yeast Cdc42 Effectors. Mol Biol Cell 18(12):4945-56
Abstract: Monitoring Editor: Martin A. Schwartz The Rho-type GTPase Cdc42 is a central regulator of eukaryotic cell polarity and signal transduction. In budding yeast, Cdc42 regulates polarity and MAP kinase signaling in part through the PAK-family kinase Ste20. Activation of Ste20 requires a Cdc42/Rac interactive binding (CRIB) domain, which mediates its recruitment to membrane-associated Cdc42. Here, we identify a separate domain in Ste20 that interacts directly with membrane phospholipids and is critical for its function. This short region, termed the basic-rich (BR) domain, can target GFP to the plasma membrane in vivo and binds PIP2-containing liposomes in vitro. Mutation of basic or hydrophobic residues in the BR domain abolishes polarized localization of Ste20 and its function in both MAP kinase-dependent and independent pathways. Thus, Cdc42 binding is required but is insufficient; instead, direct membrane binding by Ste20 is also required. Nevertheless, phospholipid specificity is not essential in vivo, as the BR domain can be replaced with several heterologous lipid-binding domains of varying lipid preferences. We also identify functionally important BR domains in two other yeast Cdc42 effectors, Gic1 and Gic2, suggesting that cooperation between protein-protein and protein-membrane interactions is a prevalent mechanism during Cdc42-regulated signaling, and perhaps for other dynamic localization events at the cell cortex.
| Status: Published | Type: Journal Article | PubMed ID: 17914055 |
Topics addressed in this paper
Number of different genes curated to this paper: 10
- To find other papers on a gene and topic, click on the colored ball in the appropriate box.
- displays other papers with information about that topic for that gene.
- displays other papers in SGD that are associated with that topic.
The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
- To go to the Locus page for a gene, click on the gene name.
| Topics | Genes linked to topics | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CDC42 | CLA4 | GIC1 | GIC2 | OPI1 | SKM1 | SPO20 | STE20 | STE4 | STE5 | |
| Additional Literature |  | | | | | | ||||
| Cellular Location | | |||||||||
| Function/Process | | | | |||||||
| Genetic Interactions | | | | | ||||||
| Mutants/Phenotypes | | | | | | |||||
| Primary Literature | | | | | ||||||
| Protein Sequence Features | | | | | | | | |||
| Protein-protein Interactions | | | ||||||||
| Regulation of | | |||||||||
| Regulatory Role | | |||||||||
| Strains/Constructs | | | | | | | ||||
