Jimeno-Gonzalez S, et al. (2006) A Gene-Specific Requirement for FACT during Transcription Is Related to the Chromatin Organization of the Transcribed Region. Mol Cell Biol 26(23):8710-8721
Abstract: The FACT complex stimulates transcription elongation on nucleosomal templates. In vivo experiments also involve FACT in the reassembly of nucleosomes traversed by RNA polymerase II. Since several features of chromatin organization vary throughout the genome, we wondered whether FACT is equally required for all genes. We show in this study that the in vivo depletion of Spt16 -one of the subunits of yeast-FACT- strongly affects transcription of three genes, GAL1, PHO5 and K. lactis LAC4, which exhibit positioned nucleosomes at their transcribed regions. In contrast, and showing a random nucleosome structure, YAT1 and E. coli lacZ are only mildly influenced by Spt16 depletion. We also show that the effect of Spt16 depletion on GAL1 expression is suppressed by a histone mutation and that the insertion of a GAL1 fragment, which allows the positioning of two nucleosomes, at the 5' end of YAT1 makes the resulting transcription unit sensitive to Spt16 depletion. These results indicate that FACT requirement for transcription depends on the chromatin organization of the 5' end of the transcribed region.
|Status: Published||Type: Journal Article||PubMed ID: 17000768|
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