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Acker MG, et al.  (2006) Interaction between eukaryotic initiation factors 1A and 5B is required for efficient ribosomal subunit joining. J Biol Chem 281(13):8469-75

Abstract: Eukaryotic initiation factor 5B (eIF5B) is a GTPase that facilitates joining of the 60 S ribosomal subunit to the 40 S ribosomal subunit during translation initiation. Formation of the resulting 80 S initiation complex triggers eIF5B to hydrolyze its bound GTP, reducing the affinity of the factor for the complex and allowing it to dissociate. Here we present a kinetic analysis of GTP hydrolysis by eIF5B in the context of the translation initiation pathway. Our data indicate that stimulation of GTP hydrolysis by eIF5B requires the completion of early steps in translation initiation, including the eIF1- and eIF1A-dependent delivery of initiator methionyl-tRNA to the 40 S ribosomal subunit and subsequent GTP hydrolysis by eIF2. Full activation of GTP hydrolysis by eIF5B requires the extreme C terminus of eIF1A, which has previously been shown to interact with the C terminus of eIF5B. Disruption of either isoleucine residue in the eIF1A C-terminal sequence DIDDI reduces the rate constant for GTP hydrolysis by approximately 20-fold, whereas changing the aspartic acid residues has no effect. Changing the isoleucines in the C terminus of eIF1A also disrupts the ability of eIF5B to facilitate subunit joining. These data indicate that the interaction of the C terminus of eIF1A with eIF5B promotes ribosomal subunit joining and possibly provides a checkpoint for correct complex formation, allowing full activation of GTP hydrolysis only upon formation of a properly organized 80 S initiation complex.

Status: Published Type: Journal Article | Research Support, N.I.H., Intramural | Research Support, Non-U.S. Gov't PubMed ID: 16461768

Topics addressed in this paper

Number of different genes curated to this paper: 100

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Topics Genes linked to topics (#1 - 10 )
ARX1 ASC1 FUN12 GCD11 JJJ1 NIP7 NMD3 RDN18-1 RDN18-2 RDN25-1
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Topics Genes linked to topics (#11 - 20 )
RDN25-2 RDN5-1 RDN5-2 RDN5-3 RDN5-4 RDN5-5 RDN5-6 RDN58-1 RDN58-2 REH1
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Topics Genes linked to topics (#21 - 30 )
REI1 RPL10 RPL11A RPL11B RPL12A RPL12B RPL13A RPL13B RPL14A RPL14B
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Topics Genes linked to topics (#31 - 40 )
RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A RPL18B RPL19A RPL19B
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Topics Genes linked to topics (#41 - 50 )
RPS0A RPS0B RPS10A RPS10B RPS11A RPS11B RPS12 RPS13 RPS14A RPS14B
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Topics Genes linked to topics (#51 - 60 )
RPS15 RPS16A RPS16B RPS17A RPS17B RPS18A RPS18B RPS19A RPS19B RPS1A
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Topics Genes linked to topics (#61 - 70 )
RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B RPS23A RPS23B RPS24A
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Topics Genes linked to topics (#71 - 80 )
RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A
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Topics Genes linked to topics (#81 - 90 )
RPS29B RPS3 RPS30A RPS30B RPS31 RPS4A RPS4B RPS5 RPS6A RPS6B
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Topics Genes linked to topics (#91 - 100 )
RPS7A RPS7B RPS8A RPS8B RPS9A RPS9B SUI1 SUI2 SUI3 TIF11
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