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Feng W, et al.  (2006) Genomic mapping of single-stranded DNA in hydroxyurea-challenged yeasts identifies origins of replication. Nat Cell Biol 8(2):148-55

Abstract: During DNA replication one or both strands transiently become single stranded: first at the sites where initiation of DNA synthesis occurs (known as origins of replication) and subsequently on the lagging strands of replication forks as discontinuous Okazaki fragments are generated. We report a genome-wide analysis of single-stranded DNA (ssDNA) formation in the presence of hydroxyurea during DNA replication in wild-type and checkpoint-deficient rad53 Saccharomyces cerevisiae cells. In wild-type cells, ssDNA was first observed at a subset of replication origins and later 'migrated' bi-directionally, suggesting that ssDNA formation is associated with continuously moving replication forks. In rad53 cells, ssDNA was observed at virtually every known origin, but remained there over time, suggesting that replication forks stall. Telomeric regions seemed to be particularly sensitive to the loss of Rad53 checkpoint function. Replication origins in Schizosaccharomyces pombe were also mapped using our method.

Status: Published Type: Journal Article PubMed ID: 16429127

Topics addressed in this paper

Number of different genes curated to this paper: 34

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
ARS1202 ARS1207 ARS1219 ARS1230 ARS1319 ARS1322 ARS1333 ARS1335 ARS1410 ARS1416
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DNA/RNA Sequence Features yg ball
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Topics Genes linked to topics (#11 - 20 )
ARS1512 ARS1518 ARS1608 ARS1612 ARS1617 ARS1620 ARS1632 ARS411 ARS412 ARS415
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Topics Genes linked to topics (#21 - 30 )
ARS420 ARS436 ARS439 ARS709 ARS712 ARS715 ARS723 ARS735 ARS801 ARS806
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Topics Genes linked to topics (#31 - 34 )
ARS815 ARS904 ARS915 RAD53
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