Bouchoux C, et al. (2004) CTD kinase I is involved in RNA polymerase I transcription. Nucleic Acids Res 32(19):5851-60
Abstract: RNA polymerase II carboxy terminal domain (CTD) kinases are key elements in the control of mRNA synthesis. Yeast CTD kinase I (CTDK-I), is a non-essential complex involved in the regulation of mRNA synthesis at the level of transcription elongation, pre-mRNA 3' formation and nuclear export. Here, we report that CTDK-I is also involved in ribosomal RNA synthesis. We show that CTDK-I is localized in part in the nucleolus. In its absence, nucleolar structure and RNA polymerase I transcription are affected. In vitro experiments show an impairment of the Pol I transcription machinery. Remarkably, RNA polymerase I co-precipitates from cellular extracts with Ctk1, the kinase subunit of the CTDK-I complex. In vitro analysis further demonstrates a direct interaction between RNA polymerase I and Ctk1. The results suggest that CTDK-I might participate in the regulation of distinct nuclear transcriptional machineries, thus playing a role in the adaptation of the global transcriptional response to growth signalling.
|Status: Published||Type: Journal Article | Research Support, Non-U.S. Gov't||PubMed ID: 15520468|
Topics addressed in this paper
Number of different genes curated to this paper: 19
- To go to the Locus page for a gene, click on the gene name.
|Topics||Genes (#1 - 10 )|
|RNA Levels and Processing|
|Topics||Genes (#11 - 19 )|