Lee SY, et al. (2003) Microbial cell-surface display. Trends Biotechnol 21(1):45-52
Abstract: Cell-surface display allows peptides and proteins to be displayed on the surface of microbial cells by fusing them with the anchoring motifs. The protein to be displayed - the passenger protein - can be fused to an anchoring motif - the carrier protein - by N-terminal fusion, C-terminal fusion or sandwich fusion. The characteristics of carrier protein, passenger protein and host cell, and fusion method all affect the efficiency of surface display of proteins. Microbial cell-surface display has many potential applications, including live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption.
|Status: Published||Type: Journal Article | Research Support, Non-U.S. Gov't | Review||PubMed ID: 12480350|
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