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Stevens SW, et al.  (2001) Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae. RNA 7(11):1543-53

Abstract: We have purified the yeast U5 and U6 pre-mRNA splicing small nuclear ribonucleoproteins (snRNPs) by affinity chromatography and analyzed the associated polypeptides by mass spectrometry. The yeast U5 snRNP is composed of the two variants of U5 snRNA, six U5-specific proteins and the 7 proteins of the canonical Sm core. The U6 snRNP is composed of the U6 snRNA, Prp24, and the 7 Sm-Like (LSM) proteins. Surprisingly, the yeast DEAD-box helicase-like protein Prp28 is stably associated with the U5 snRNP, yet is absent from the purified U4/U6 x U5 snRNP. A novel yeast U5 and four novel yeast U4/U6 x U5 snRNP polypeptides were characterized by genetic and biochemical means to demonstrate their involvement in the pre-mRNA splicing reaction. We also show that, unlike the human tri-snRNP, the yeast tri-snRNP dissociated upon addition of ATP or dATP.

Status: Published Type: Journal Article PubMed ID: 11720284

Topics addressed in this paper

Number of different genes curated to this paper: 27

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Topics Genes linked to topics (#1 - 10 )
BRR2 DIB1 LIN1 LSM2 LSM3 LSM4 LSM5 LSM6 LSM7 LSM8
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Topics Genes linked to topics (#11 - 20 )
PRP24 PRP28 PRP8 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3
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Topics Genes linked to topics (#21 - 27 )
SNR6 SNR7-L SNR7-S SNU114 SNU13 SNU23 SNU66
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