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Suka N, et al.  (2001) Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin. Mol Cell 8(2):473-9

Abstract: We have developed a highly specific antibody set for acetylation sites in yeast histones H4 (K5, K8, K12, and K16); H3 (K9, K14, K18, K23, and K27); H2A (K7); and H2B (K11 and K16). Since ELISA does not assure antibody specificity in chromatin immunoprecipitation, we have employed additional screens against the respective histone mutations. We now show that telomeric and silent mating locus heterochromatin is hypoacetylated at all histone sites. At the INO1 promoter, RPD3 is required for strongly deacetylating all sites except H4 K16, ESA1 for acetylating H2A, H2B, and H4 sites except H4 K16, and GCN5 for acetylating H2B and H3 sites except H3 K14. These data uncover the in vivo usage of acetylation sites in heterochromatin and euchromatin.

Status: Published Type: Clinical Conference | Journal Article PubMed ID: 11545749

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Topics Genes linked to topics (#1 - 10 )
ESA1 GCN5 HHF1 HHF2 HHT1 HHT2 HTA1 HTA2 HTB1 HTB2
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Topics Genes linked to topics (#11 - 12 )
INO1 RPD3
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