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Merkulov GV, et al.  (2001) Ty1 proteolytic cleavage sites are required for transposition: all sites are not created equal. J Virol 75(2):638-44

Abstract: The retroviral protease is a key enzyme in a viral multienzyme complex that initiates an ordered sequence of events leading to virus assembly and propagation. Viral peptides are initially synthesized as polyprotein precursors; these precursors undergo a number of proteolytic cleavages executed by the protease in a specific and presumably ordered manner. To determine the role of individual protease cleavage sites in Ty1, a retrotransposon from Saccharomyces cerevisiae, the cleavage sites were systematically mutagenized. Altering the cleavage sites of the yeast Ty1 retrotransposon produces mutants with distinct retrotransposition phenotypes. Blocking the Gag/PR site also blocks cleavage at the other two cleavage sites, PR/IN and IN/RT. In contrast, mutational block of the PR/IN or IN/RT sites does not prevent cleavage at the other two sites. Retrotransposons with mutations in each of these sites have transposition defects. Mutations in the PR/IN and IN/RT sites, but not in the Gag/PR site, can be complemented in trans by endogenous Ty1 copies. Hence, the digestion of the Gag/PR site and release of the protease N terminus is a prerequisite for processing at the remaining sites; cleavage of PR/IN is not required for the cleavage of IN/RT, and vice versa. Of the three cleavage sites in the Gag-Pol precursor, the Gag/PR site is processed first. Thus, Ty1 Gag-Pol processing proceeds by an ordered pathway.

Status: Published Type: Journal Article PubMed ID: 11134277

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Topics Genes linked to topics (#1 - 10 )
YAR009C YARCTy1-1 YBL005W-B YBL100W-B YBLWTy1-1 YBLWTy2-1 YBR012W-B YBRWTy1-2 YCL019W YCLWTy2-1
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Topics Genes linked to topics (#11 - 20 )
YDR034C-D YDR098C-B YDR210C-D YDR210W-B YDR261C-D YDR261W-B YDR316W-B YDR365W-B YDRCTy1-1 YDRCTy1-2
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Topics Genes linked to topics (#21 - 30 )
YDRCTy1-3 YDRCTy2-1 YDRWTy1-4 YDRWTy1-5 YDRWTy2-2 YDRWTy2-3 YER138C YER160C YERCTy1-1 YERCTy1-2
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Topics Genes linked to topics (#31 - 40 )
YFL002W-A YFLWTy2-1 YGR027W-B YGR038C-B YGR161C-D YGR161W-B YGRCTy1-2 YGRCTy1-3 YGRCTy2-1 YGRWTy1-1
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Topics Genes linked to topics (#41 - 50 )
YGRWTy2-2 YHR214C-B YHRCTy1-1 YJR027W YJR029W YJRWTy1-1 YJRWTy1-2 YLR035C-A YLR157C-B YLR256W-A
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Topics Genes linked to topics (#51 - 60 )
YLR410W-B YLRCTy1-1 YLRCTy2-2 YLRWTy1-2 YLRWTy1-3 YLRWTy2-1 YML039W YML045W YMLWTy1-1 YMLWTy1-2
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Topics Genes linked to topics (#61 - 70 )
YMR045C YMR050C YMRCTy1-3 YMRCTy1-4 YNL054W-B YNL284C-B YNLCTy1-1 YNLCTy2-1 YNLWTy1-2 YOL103W-B
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Topics Genes linked to topics (#71 - 80 )
YOLWTy1-1 YOR142W-B YOR192C-B YOR343W-B YORCTy2-1 YORWTy1-2 YORWTy2-2 YPL257W-B YPLWTy1-1 YPR137C-B
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Topics Genes linked to topics (#81 - 85 )
YPR158C-D YPR158W-B YPRCTy1-2 YPRCTy1-4 YPRWTy1-3
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