SGD Paper Help


Page Contents:
Jin H and Amberg DC  (2000) The secretory pathway mediates localization of the cell polarity regulator Aip3p/Bud6p. Mol Biol Cell 11(2):647-61

Abstract: Aip3p/Bud6p is a regulator of cell and cytoskeletal polarity in Saccharomyces cerevisiae that was previously identified as an actin-interacting protein. Actin-interacting protein 3 (Aip3p) localizes at the cell cortex where cytoskeleton assembly must be achieved to execute polarized cell growth, and deletion of AIP3 causes gross defects in cell and cytoskeletal polarity. We have discovered that Aip3p localization is mediated by the secretory pathway. Mutations in early- or late-acting components of the secretory apparatus lead to Aip3p mislocalization. Biochemical data show that a pool of Aip3p is associated with post-Golgi secretory vesicles. An investigation of the sequences within Aip3p necessary for Aip3p localization has identified a sequence within the N terminus of Aip3p that is sufficient for directing Aip3p localization. Replacement of the N terminus of Aip3p with a homologous region from a Schizosaccharomyces pombe protein allows for normal Aip3p localization, indicating that the secretory pathway-mediated Aip3p localization pathway is conserved. Delivery of Aip3p also requires the type V myosin motor Myo2p and its regulatory light-chain calmodulin. These data suggest that one function of calmodulin is to activate Myo2p's activity in the secretory pathway; this function is likely the polarized movement of late secretory vesicles and associated Aip3p on actin cables.

Status: Published Type: Journal Article PubMed ID: 10679021

Topics addressed in this paper

Number of different genes curated to this paper: 9

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
BNI1 BUD6 CDC42 CMD1 MYO2 SEC15 SEC17 SEC4 SEC6
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball
Cellular Location blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball
Protein Sequence Features blue ball
Protein-protein Interactions blue ball
Protein/Nucleic Acid Structure blue ball
Regulation of blue ball
Regulatory Role blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: