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Reeder RH, et al.  (1999) Saccharomyces cerevisiae RNA polymerase I terminates transcription at the Reb1 terminator in vivo. Mol Cell Biol 19(11):7369-76

Abstract: We have mapped transcription termination sites for RNA polymerase I in the yeast Saccharomyces cerevisiae. S1 nuclease mapping shows that the primary terminator is the Reb1p terminator located at +93 downstream of the 3' end of 25S rRNA. Reverse transcription coupled with quantitative PCR shows that approximately 90% of all transcripts terminate at this site. Transcripts which read through the +93 site quantitatively terminate at a fail-safe terminator located further downstream at +250. Inactivation of Rnt1p (an RNase III involved in processing the 3' end of 25S rRNA) greatly stabilizes transcripts extending to both sites and increases readthrough at the +93 site. In vivo assay of mutants of the Reb1p terminator shows that this site operates in vivo by the same mechanism as has previously been delineated through in vitro studies.

Status: Published Type: Journal Article PubMed ID: 10523625

Topics addressed in this paper

Number of different genes curated to this paper: 19

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Topics Genes linked to topics (#1 - 10 )
ETS2-1 ETS2-2 RDN1 REB1 RNT1 RPA12 RPA135 RPA14 RPA190 RPA34
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball
DNA/RNA Sequence Features blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball
Primary Literature blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball
RNA Levels and Processing blue ball
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Transcription blue ball blue ball blue ball

Topics Genes linked to topics (#11 - 19 )
RPA43 RPA49 RPB10 RPB5 RPB8 RPC10 RPC19 RPC40 RPO26
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

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