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Rigaut G, et al.  (1999) A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol 17(10):1030-2

Abstract: We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.

Status: Published Type: Journal Article PubMed ID: 10504710

Topics addressed in this paper

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Topics Genes linked to topics (#1 - 10 )
CBC2 MAK10 MAK3 MAK31 MUD1 NAM8 PRP39 PRP40 PRP42 SMD1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#11 - 18 )
SMD2 SMD3 SNP1 SNU56 SNU71 SRP1 STO1 YHC1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Techniques and Reagents blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

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