AFR1/YDR085C Protein Information Help

AFR1 BASIC PROTEIN INFORMATION

Standard Name Afr1p
Systematic Name Ydr085cp
ORF Classification Verified
Description Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p ( 1, 2, 3, 4, 5 )
Name Description Alpha-Factor Receptor regulator 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 620
Molecular Weight (Da) 71,363
Isoelectric Point (pI) 9.23

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Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Afr1p (InterPro)
Physical Interactions There are 16 total curated physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | PSI-BLAST | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
Secondary Structure Prediction GCG:PepPlot | GCG:PepStruct | GCG:Helical Wheel
External Sequence Databases EBI: P33304 | UPI0000125676
MIPS: YDR085C
NCBI: NP_010370.1 | 6320290
GenBank/EMBL/DDBJ: AY723775
Amino Acid Sequence (or in FASTA format)
 
       1  MEGSYLSAQE NQPIPERLIP RSNSTSNLFA LSSTFSKLNV RNDADYNYSN
      51  PNKKRHIYSG EIDCRSVTAA RKFPVRSCSM TAAQQRKRTA LFTVRERNSY
     101  HEGFNNDQDY VSQYQKPQYT FGVYKELTPY QLQRSKMKRS FQFPNGEIYK
     151  PKLDGKCTHS LKKPELNSRD SSLFKFSEKK GRNLSKDFVG PHNGTSVIHI
     201  PPNDTGYGVN SLELNTSVPS TIKSSVSSTS PISAVNTLTS LPESQTDDDD
     251  GYENKTVTIS YCFENTVNEK HGSHIEKLDL STKEKTKPTT NSGLFDRKKK
     301  TILGTEKYRC IKSQSKLKLG SVLKKLWRTS GNSNTKHGKK DTKRRRIPID
     351  DMVTHSDGNS EAENDIELMD ANLDGIEFDD DETLMDTDSI FDDLLSKEND
     401  KYDLRRRQLE IRQKLHETSH NDDGKVSFRD TEKHNVNEGL IDKTIIEEFS
     451  KLGEYIIDTR NQPPPRSSKR PSLDDNESAR YFYNISTDLR QSLSGPISLP
     501  MHVGNDMVNR LRNDWEYIRF EDRRNSLPDS SFDKVETPPK PIKKDVRFAK
     551  EVCLASTWSS NAYERANPEF IMNRHRLLWM MKVHPSMNSA MNEIKLELNS
     601  YKKNEMVVHE NSKCFTHYLI *

EXTERNAL LINKS for Afr1p

Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YGAC Triples
Ashbya (AGD) BOND GPMdb (Mass Spec.)
YGOB BioPIXIE MIPS
YOGY CYC2008 (complexes) Pfam domains

DIP PhosphoPep Database

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for Afr1p ]

1) DeMattei CR, et al.  (2000) Point mutations identify a conserved region of the saccharomyces cerevisiae AFR1 gene that is essential for both the pheromone signaling and morphogenesis functions. Genetics 155(1):43-55
2) Konopka JB  (1993) AFR1 acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation. Mol Cell Biol 13(11):6876-88
3) Giot L and Konopka JB  (1997) Functional analysis of the interaction between Afr1p and the Cdc12p septin, two proteins involved in pheromone-induced morphogenesis. Mol Biol Cell 8(6):987-98
4) Changwei Z, et al.  (2007) Afr1p has a role in regulating the localization of Mpk1p at the shmoo tip in Saccharomyces cerevisiae. FEBS Lett 581(14):2670-4
5) Bharucha JP, et al.  (2008) Saccharomyces cerevisiae Afr1 protein is a protein phosphatase 1/Glc7-targeting subunit that regulates the septin cytoskeleton during mating. Eukaryot Cell 7(8):1246-55
6) Davis C, et al.  (1998) Afr1p regulates the Saccharomyces cerevisiae alpha-factor receptor by a mechanism that is distinct from receptor phosphorylation and endocytosis. Genetics 148(2):625-35